From Metabolonote
jump-to-nav Jump to: navigation, search

Sample Set Information

Title A novel method for single-grain-based metabolic profiling of Arabidopsis seed
Description In plant metabolomics, metabolite contents are often normalized by sample weight. However, accurate weighing of very small samples, such as individual Arabidopsis thaliana seeds (approximately 20 µg), is difficult, which may lead to irreproducible results.
Authors Yuji Sawada, Hirokazu Tsukaya, Yimeng Li, Muneo Sato, Kensuke Kawade, Masami Yokota Hirai
Reference Sawada et al. (2017) Metabolomics 13:75

Link icon article.png

Link icon database.png Link icon dropmet.png

The raw data files are available at DROP Met web site in PRIMe database of RIKEN.

Sample Information

Title Arabidopsis thaliana seeds
Organism - Scientific Name Arabidopsis thaliana
Organism - ID NCBI taxonomy 3702
Compound - ID
Compound - Source
Preparation Tetraploidization of A. thaliana was performed in an accession Col-0, as described previously (Breuer et al. 2007), and the ploidy type was confirmed by flow cytometry analysis (Kozuka et al. 2005). Diploid and autotetraploid plants were maintained at 23 °C under continuous illumination (ca. 60 µmol/sec/m2) on rockwool supplied with 0.2 g/L HYPONeX solution, as described elsewhere (Fujikura et al. 2007). To harvest mature seeds for metabolomic analysis, 1-week-old plants grown on agar-solidified, half-strength Murashige and Skoog medium were transferred to soil [PRO-MIX (Premier Horticulture, Canada): vermiculite = 2:1] and grown under 16 h light/8 h dark with a nutrient solution reported as a control medium in Hirai et al. (1995). For biological replicates, seeds were independently collected from 12 individuals for each genotype (Supplementary Table S1).
Sample Preparation Details ID

Personal tools
View and Edit Metadata