SE131:/MS2

From Metabolonote
jump-to-nav Jump to: navigation, search

Sample Set Information

ID TSE16
Title Integrated LC-MS/MS system for plant metabolomics
Description Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is highly sensitive, selective, and enables extensive detection of metabolites within a sample. The result allows us to characterize comprehensive metabolite accumulation patterns without dependence on authentic standard compounds and isolation of the individual metabolites. A reference database search is essential for the structural assignment process of un-targeted MS and MS/MS data. Moreover, the characterization of unknown metabolites is challenging, since these cannot be assigned a candidate structure by using a reference database. In this case study, integrated LC-MS/MS based plant metabolomics allows us to detect several hundred metabolites in a sample; and integrated omics analyses, e.g., large-scale reverse genetics, linkage mapping, and association mapping, provides a powerful tool for candidate structure selection or rejection. We also examine emerging technology and applications for LC-MS/MS-based un-targeted plant metabolomics. These activities promote the characterization of massive extended detectable metabolites.
Authors Yuji Sawada, Masami Yokota Hirai
Reference Sawada and Hirai (2013) Computational and Structural Biotechnology Journal 23;4:e201301011. doi: 10.5936/csbj.201301011. eCollection 2013
Comment


Link icon article.png

Link icon database.png Link icon dropmet.png

The raw data files are available at DROP Met web site in PRIMe database of RIKEN.

Analytical Method Details Information

ID
Title
Instrument
Instrument Type
Ionization
Ion Mode
Description
Comment_of_details


Personal tools
View and Edit Metadata
Variants
Views
Actions