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Sample Set Information

ID SE137
Title Metabolite Signature during Short-Day Induced Growth Cessation in Populus.
Description The photoperiod is an important environmental signal for plants, and influences a wide range of physiological processes. For woody species in northern latitudes, cessation of growth is induced by short photoperiods. In many plant species, short photoperiods stop elongational growth after a few weeks. It is known that plant daylength detection is mediated by Phytochrome A (PHYA) in the woody hybrid aspen species. However, the mechanism of dormancy involving primary metabolism remains unclear. We studied changes in metabolite profiles in hybrid aspen leaves (young, middle, and mature leaves) during short-day-induced growth cessation, using a combination of gas chromatography–time-of-flight mass spectrometry, and multivariate projection methods. Our results indicate that the metabolite profiles in mature source leaves rapidly change when the photoperiod changes. In contrast, the differences in young sink leaves grown under long and short-day conditions are less distinct. We found short daylength induced growth cessation in aspen was associated with rapid changes in the distribution and levels of diverse primary metabolites. In addition, we conducted metabolite profiling of leaves of PHYA overexpressor (PHYAOX) and those of the control to find the discriminative metabolites between PHYAOX and the control under the short-day conditions. The metabolite changes observed in PHYAOX leaves, together with those in the source leaves, identified possible candidates for the metabolite signature (e.g., 2-oxo-glutarate, spermidine, putrescine, 4-amino-butyrate, and tryptophan) during short-day-induced growth cessation in aspen leaves.
Authors Kusano M, Jonsson P, Fukushima A, Gullberg J, Sjöström M, Trygg J, Moritz T.
Reference Front Plant Sci. 2011 Jul 12;2:29. doi: 10.3389/fpls.2011.00029. eCollection 2011.

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Sample Information

Title hybrid aspen (P. tremula × P. tremuloides)
Organism - Scientific Name Populus tremula × Populus tremuloides
Organism - ID NCBI taxonomy 47664
Compound - ID
Compound - Source
Preparation Twenty-eight hybrid aspen trees were grown under long photoperiods (long-day conditions, LDs) of 18 h/6 h day/night cycle using photosynthetic active radiation (PAR) light for 12 h at 400 μmol m−2s−1, and extended for another 6 h at 30 μmol m−2s−1. After 3 months, 20 consecutive leaves (the length of the first leaf below the apex was approximately 1 cm) and the apex (defined as apical tissue from which all major leaf primordia had been removed) were sampled from seven plants (LD0-samples). Two days later another seven plants were sampled (LD2-samples), and the daylength was changed to short winter photoperiods (short-day conditions, SDs): 12/12 h day/night). After 2 and 6 days under short photoperiods (SD2 and SD6), seven plants were sampled.

To examine the effect of 35S: : oat PHYA-overexpression, we sampled 10 leaves from 22-PHYAOX and 21-control plants. We used two different photoperiods [LD0 and short photoperiod at day 7 (SD7)] to obtain PHYOX and the control plants (wild-type, WT). After removal from the plant samples were dipped in liquid nitrogen and stored at −80°C until required.

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