SE138:/MS3

Sample Set Information

ID	TSE1239
Title	Effects of molybdenum deficiency and defects in molybdate transporter MOT1 on transcript accumulation and nitrogen/sulphur metabolism in Arabidopsis thaliana.
Description	Molybdenum (Mo) is a micronutrient essential for plant growth, as several key enzymes of plant metabolic pathways contain Mo cofactor in their catalytic centres. Mo-containing oxidoreductases include nitrate reductase, sulphite oxidase, xanthine dehydrogenase, and aldehyde oxidase. These are involved in nitrate assimilation, sulphite detoxification, purine metabolism or the synthesis of abscisic acid, auxin and glucosinolates in plants. To understand the effects of Mo deficiency and a mutation in a molybdate transporter, MOT1, on nitrogen and sulphur metabolism in Arabidopsis thaliana, transcript and metabolite profiling of the mutant lacking MOT1 was conducted in the presence or absence of Mo. Transcriptome analysis revealed that Mo deficiency had impacts on genes involved in metabolisms, transport, stress responses, and signal transductions. The transcript level of a nitrate reductase NR1 was highly induced under Mo deficiency in mot1-1. The metabolite profiles were analysed further by using gas chromatography time-of-flight mass spectrometry, capillary electrophoresis time-of-flight mass spectrometry, and ultra high performance liquid chromatography. The levels of amino acids, sugars, organic acids, and purine metabolites were altered significantly in the Mo-deficient plants. These results are the first investigation of the global effect of Mo nutrition and MOT1 on plant gene expressions and metabolism.
Authors	Ide Y, Kusano M, Oikawa A, Fukushima A, Tomatsu H, Saito K, Hirai MY, Fujiwara T.
Reference	J Exp Bot. 2011 Feb;62(4):1483-97. doi: 10.1093/jxb/erq345. Epub 2010 Dec 3.
Comment

PubMed

Analytical Method Details Information

ID	MS3
Title	Analysis of Mo concentration
Instrument
Instrument Type
Ionization
Ion Mode
Description	Whole shoots were harvested and their Mo concentration was determined by inductively coupled plasma mass spectrometry (ICP-MS) as described previously (Takano et al., 2001, 2002). For the measurement of the inorganic ion concentrations, whole shoots were harvested and frozen in liquid nitrogen before extraction. Frozen tissues (approximately 50 mg fresh weight) were homogenized in ten times volumes of 80% ethanol using the mixer mill MM300 (Retsch, Haan, Germany) and zirconia beads, and vacuum-evaporated at 38 °C to dryness. The dried samples were reconstituted in ten times volumes of milli-Q water (Millipore, Billerica, MA), vortexed for 10 min, and ultrafiltrated through a 0.22 μm pore filter (Millipore, Billerica, MA). The filtrates were diluted 10-fold in milli-Q water and the ion concentrations were determined using a Hewlett-Packard HP 3D capillary electrophoresis photodiode array detection system and an inorganic anion analysis kit (Agilent Technologies, Santa Clara, CA) according to the manufacturer's protocol. Four biological replicates for each treatment were used for analysis.
Comment_of_details