SE150:/DS1

Sample Set Information

ID	TSE1305
Title	A new class of plant lipid is essential for protection against phosphorus depletion.
Description	Phosphorus supply is a major factor responsible for reduced crop yields. As a result, plants utilize various adaptive mechanisms against phosphorus depletion, including lipid remodelling. Here we report the involvement of a novel plant lipid, glucuronosyldiacylglycerol, against phosphorus depletion. Lipidomic analysis of Arabidopsis plants cultured in phosphorus-depleted conditions revealed inducible accumulation of glucuronosyldiacylglycerol. Investigation using a series of sulfolipid sulfoquinovosyldiacylglycerol synthesis-deficient mutants of Arabidopsis determined that the biosynthesis of glucuronosyldiacylglycerol shares the pathway of sulfoquinovosyldiacylglycerol synthesis in chloroplasts. Under phosphorus-depleted conditions, the Arabidopsis sqd2 mutant, which does not accumulate either sulfoquinovosyldiacylglycerol or glucuronosyldiacylglycerol, was the most severely damaged of three sulfoquinovosyldiacylglycerol-deficient mutants. As glucuronosyldiacylglycerol is still present in the other two mutants, this result indicates that glucuronosyldiacylglycerol has a role in the protection of plants against phosphorus limitation stress. Glucuronosyldiacylglycerol was also found in rice, and its concentration increased significantly following phosphorus limitation, suggesting a shared physiological significance of this novel lipid against phosphorus depletion in plants.
Authors	Okazaki Y, Otsuki H, Narisawa T, Kobayashi M, Sawai S, Kamide Y, Kusano M, Aoki T, Hirai MY, Saito K.
Reference	Nat Commun. 2013;4:1510. doi: 10.1038/ncomms2512.
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PubMed

Data Analysis Details Information

ID	DS1
Title	Data processing and analysis
Description	Data processing Peak picking and alignment were performed using the Profiling Solution software (version 1.0.76.0; Shimadzu). The profiling solution parameters were as follows: ion m/z tolerance, 20 mDa; ion RT tolerance, 0.1 min; ion intensity threshold, 2e4; detect isomer valley, 20%; allow some ion without isotope peak, ON. The data matrix containing ~480 ions consisting of negatively detected ions was exported from the profiling solution software and normalized relative to the intensity of the [M+HCO2]− ion of the internal standard (1,2-didecanoyl-glycero-3-phosphocholine). Annotation was based on the theoretical m/z values of each lipid species and the retention time of identified compounds with the same polar head. Statistics for the analysis of lipidomic data Normalized LC-MS data were pareto-scaled and subjected to OPLS-DA using SIMCA-P (version 11.0.0.0; Umetrics). Tukey' s test and Welch' s t-test were performed using their respective functions (TukeyHSD and t-test) in the statistical software R version 2.14.0 ( http://www.r-project.org/). HCL and jackknife testing were performed on MultiExperiment Viewer (MeV) version 4.8 ( http://www.tm4.org/mev/). For HCL, Pearson’s correlations were used to analyse the distance metric. Average linkage clustering was selected for the linkage method. The data set used for HCL is available as ‘Supplementary Data Set.xls’. Metadata also can be found in the same file.
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