SE164:/MS2
From Metabolonote
Sample Set Information
| ID | TSE1326 |
|---|---|
| Title | Omics-based approaches to methionine side chain elongation in Arabidopsis: characterization of the genes encoding methylthioalkylmalate isomerase and methylthioalkylmalate dehydrogenase. |
| Description | Glucosinolates (GSLs) are secondary metabolites in Brassicaceae plants synthesized from amino acids. Methionine-derived GSLs (Met-GSLs) with diverse side chains of various lengths are the major GSLs in Arabidopsis. Methionine chain elongation enzymes are responsible for variations in chain length in Met-GSL biosynthesis. The genes encoding methionine chain elongation enzymes are considered to have been recruited from the leucine biosynthetic pathway in the course of evolution. Among them, the genes encoding methylthioalkylmalate synthases and aminotransferases have been identified; however, the remaining genes that encode methylthioalkylmalate isomerase (MAM-I) and methylthioalkylmalate dehydro-genase (MAM-D) remain to be identified. In a previous study based on transcriptome co-expression analysis, we identified candidate genes for the large subunit of MAM-I and MAM-D. In this study, we confirmed their predicted functions by targeted GSL analysis of the knockout mutants, and named the respective genes MAM-IL1/AtleuC1 and MAM-D1/AtIMD1. Metabolic profiling of the knockout mutants of methionine chain elongation enzymes, conducted by means of widely targeted metabolomics, implied that these enzymes have roles in controlling metabolism from methionine to primary and methionine-related secondary metabolites. As shown here, an omics-based approach is an efficient strategy for the functional elucidation of genes involved in metabolism. |
| Authors | Sawada Y, Kuwahara A, Nagano M, Narisawa T, Sakata A, Saito K, Hirai MY. |
| Reference | Plant Cell Physiol. 2009 Jul;50(7):1181-90. doi: 10.1093/pcp/pcp079. Epub 2009 Jun 3. |
| Comment |
Analytical Method Details Information
| ID | MS2 |
|---|---|
| Title | UPLC-TQD (Widely targeted metabolomics) |
| Instrument | UPLC-TQD system (Waters) |
| Instrument Type | UPLC-QTOF-MS |
| Ionization | ESI |
| Ion Mode | Positive and Negative |
| Description | Approximately 50–100 mg of leaves (3 weeks after germination for mam1 and mam3, and 4 weeks after germination for atleuc1 and atimd1) of wild-type and mutant Arabidopsis lines were used for widely targeted metabolomics using UPLC-TQD (Waters) as previously described (Sawada et al. 2009). Analytical conditions for UPLC-TQD are released in our data repository and distribution site DROP Met at our website PRIMe (http://prime.psc.riken.jp/). |
| Comment_of_details |
