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Sample Set Information

ID TSE1350
Title Enhanced radical scavenging activity of genetically modified Arabidopsis seeds
Description The proanthocyanidin (PA) content was increased in seeds of pap1-D mutant of Arabidopsis thaliana, in which the expression of endogenous PAP1 gene encoding a Myb-like transcription factor was induced by activation-tagging with enhancer sequences derived from cauliflower mosaic virus 35S promoter. In contrast, the PA contents decreased in seeds of transgenic plants transformed with a PAP1 cDNA or with a PAP1 chimeric repressor, although the amount of soluble anthocyanins increased in seeds of transgenic plants over-expressing PAP1cDNA. The enhanced radical scavenging activity was observed only in the seed extracts of pap1-D mutant, indicating that PAs are primarily responsible for radical scavenging activity in seeds. The present study suggests the feasibility of engineering a transcription factor of flavonoid biosynthesis for health beneficial plant seeds.
Authors Tohge, T., Matsui, K., Ohme-Takagi, M., Yamazaki, M. and Saito, K.
Reference Biotechnol Lett. 2005 Mar;27(5):297-303

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Sample Information

Title Arabidopsis thaliana
Organism - Scientific Name Arabidopsis thaliana
Organism - ID NCBI taxonomy:3702
Compound - ID
Compound - Source
Preparation The pap1-D mutant of A. thaliana was described previously (Borevitz et al. 2000). The PAP1 cDNA-over-expressing transgenic A. thaliana (PAP1OX) was obtained by transformation with the construct carrying cauliflower mosaicvirus 35S promoter linked with the coding sequence of PAP1 cDNA as described elsewhere (Tohge et al. 2005). The transgenic plant expressing PAP1 chimeric repressor (PAP1SRDX) was described previously (Hiratsu et al. 2003, Matsui et al. 2004). The plants were grown in a standard greenhouse at 22 C in 16 h light for 8 weeks. Seeds were harvested and stored at room temperature for more than 1 week for drying. These seeds were used for analysis of anthocyanins, PAs, and radical-scavenging activity.
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