SE214:/MS1

From Metabolonote
jump-to-nav Jump to: navigation, search

Sample Set Information

ID SE214
Title Solanum lycopersicum metabolite analysis
Description Investigation of Solanum lycopersicum cv. Micro-Tom metabolites. 5 tissue (Leaf, Stem, Root, Green Fruit, Red Fruit) data are examined (Jan 2005).
Authors Takeshi Ara 1, Yoshihiko Morishita 1, Fumi Maeda 1, Hideyuki Suzuki 1, Daisuke Shibata 1, 1: Kazusa DNA Research Institute
Reference Direct submission
Comment


[PGDBj] http://pgdbj.jp/plantdb/plantinfo.html?ln=en&cmd=entry&ppid=t4081

Analytical Method Details Information

ID MS1
Title GC-TOF-MS lipid fraction method
Instrument 6890N GC (Agilent Technologies); Pegasus III TOF-MS system (Leco)
Instrument Type
Ionization EI
Ion Mode Positive
Description Frozen sample was homogenized using mill mixer with liquid nitrogen. Metabolites were extracted by MeOH/H2O/CHCl3. After liquid‐liquid distribution, upper (MeOH/H2O) layer is used as polar fraction and bottom (CHCl3) layer is used as lipid fraction. Ribitol and nonadecanoic acid methyl ester were used for internal standards of polar and lipid fractions. Lipid fraction was methoximated with CHCl3/NaOH/MeOH. After washing by water, CHCl3 layer was dried over by N2 gas. Metabolites were trimethylsilylated with n-Heptane/Pyridine/MSTFA. GC conditions: Agilent 6890N GC (Agilent), Carrier gas: He, MS conditions: Pegasus III TOF-MS system (Leco), mass range: 35 to 950 m/z.
Comment_of_details Instrument Type: GC-TOF-MS


Personal tools
View and Edit Metadata
Variants
Views
Actions