SE214:/S05/M01

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Sample Set Information

ID SE214
Title Solanum lycopersicum metabolite analysis
Description Investigation of Solanum lycopersicum cv. Micro-Tom metabolites. 5 tissue (Leaf, Stem, Root, Green Fruit, Red Fruit) data are examined (Jan 2005).
Authors Takeshi Ara 1, Yoshihiko Morishita 1, Fumi Maeda 1, Hideyuki Suzuki 1, Daisuke Shibata 1, 1: Kazusa DNA Research Institute
Reference Direct submission
Comment


[PGDBj] http://pgdbj.jp/plantdb/plantinfo.html?ln=en&cmd=entry&ppid=t4081

Sample Information

ID S05
Title Solanum lycopersicum cv. Micro-Tom Stem
Organism - Scientific Name Solanum lycopersicum
Organism - ID NCBI taxonomy:4081
Compound - ID
Compound - Source
Preparation
Sample Preparation Details ID
Comment

Analytical Method Information

ID M01
Title GC-TOF-MS analysis, lipid fraction
Method Details ID MS1
Sample Amount
Comment [MassBase ID] MDGC1_04782


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The raw (binary) and near-raw (text) files of this analysis are available at MassBase.

Analytical Method Details Information

ID MS1
Title GC-TOF-MS lipid fraction method
Instrument 6890N GC (Agilent Technologies); Pegasus III TOF-MS system (Leco)
Instrument Type
Ionization EI
Ion Mode Positive
Description Frozen sample was homogenized using mill mixer with liquid nitrogen. Metabolites were extracted by MeOH/H2O/CHCl3. After liquid‐liquid distribution, upper (MeOH/H2O) layer is used as polar fraction and bottom (CHCl3) layer is used as lipid fraction. Ribitol and nonadecanoic acid methyl ester were used for internal standards of polar and lipid fractions. Lipid fraction was methoximated with CHCl3/NaOH/MeOH. After washing by water, CHCl3 layer was dried over by N2 gas. Metabolites were trimethylsilylated with n-Heptane/Pyridine/MSTFA. GC conditions: Agilent 6890N GC (Agilent), Carrier gas: He, MS conditions: Pegasus III TOF-MS system (Leco), mass range: 35 to 950 m/z.
Comment_of_details Instrument Type: GC-TOF-MS
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