SE215:/S02/M02

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Sample Set Information

ID SE215
Title Comparison of metabolites among Legume species
Description Investigation of Legume metabolites. 2 species (Medicago sativa, Medicago truncatula) and 3 varieties (B-129, Burttii, MG-20) of 1 species (Lotus japonicus) data are examined (Mar 2004).
Authors Takeshi Ara 1, Yoshihiko Morishita 1, Hideyuki Suzuki 1, Daisuke Shibata 1, 1: Kazusa DNA Research Institute
Reference Direct submission
Comment [PGDBj] http://pgdbj.jp/plantdb/plantinfo.html?ln=en&cmd=entry&ppid=t34305

[PGDBj] http://pgdbj.jp/plantdb/plantinfo.html?ln=en&cmd=entry&ppid=t3880

Sample Information

ID S02
Title Lotus burttii B-303
Organism - Scientific Name Lotus burttii
Organism - ID NCBI taxonomy:645164
Compound - ID
Compound - Source
Preparation
Sample Preparation Details ID
Comment

Analytical Method Information

ID M02
Title GC-TOF-MS analysis, lipid fraction
Method Details ID MS1
Sample Amount
Comment [MassBase ID] MDGC1_04805


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Analytical Method Details Information

ID MS1
Title GC-TOF-MS lipid fraction method
Instrument 6890N GC (Agilent Technologies); Pegasus III TOF-MS system (Leco)
Instrument Type
Ionization EI
Ion Mode Positive
Description Frozen sample was homogenized using mill mixer with liquid nitrogen. Metabolites were extracted by MeOH/H2O/CHCl3. After liquid‐liquid distribution, upper (MeOH/H2O) layer is used as polar fraction and bottom (CHCl3) layer is used as lipid fraction. Ribitol and nonadecanoic acid methyl ester were used for internal standards of polar and lipid fractions. Lipid fraction was methoximated with CHCl3/NaOH/MeOH. After washing by water, CHCl3 layer was dried over by N2 gas. Metabolites were trimethylsilylated with n-Heptane/Pyridine/MSTFA. GC conditions: Agilent 6890N GC (Agilent), Carrier gas: He, MS conditions: Pegasus III TOF-MS system (Leco), mass range: 35 to 950 m/z.
Comment_of_details Instrument Type: GC-TOF-MS
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