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Sample Set Information

ID SE217
Title Comparison of tomato fruit metabolites among fruit maturation
Description Investigation of tomato fruit metabolites. 4 varieties (Solanum lycopersicum, Solanum lycopersicum cv. Micro-Tom, Solanum pimpinellifolium, Solanum lycopersicum x Solanum pimpinellifolium) and 4 maturation stages (green, mature green, breaker, red ripe)data are examined (May 2004).
Authors Takeshi Ara 1, Fumi Maeda 1, Hideyuki Suzuki 1, Daisuke Shibata 1, 1: Kazusa DNA Research Institute
Reference Direct submission


Sample Information

ID S08
Title Solanum pimpinellifolium Fruit breaker stage
Organism - Scientific Name Solanum pimpinellifolium
Organism - ID NCBI taxonomy:4084
Compound - ID
Compound - Source
Sample Preparation Details ID

Analytical Method Information

ID M04
Title GC-TOF-MS analysis, lipid fraction
Method Details ID MS1
Sample Amount
Comment [MassBase ID] MDGC1_04172

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The raw (binary) and near-raw (text) files of this analysis are available at MassBase.

Analytical Method Details Information

Title GC-TOF-MS lipid fraction method
Instrument MS1
Instrument Type
Ionization EI
Ion Mode Positive
Description Frozen sample was homogenized using mill mixer with liquid nitrogen. Metabolites were extracted by MeOH/H2O/CHCl3. After liquid‐liquid distribution, upper (MeOH/H2O) layer is used as polar fraction and bottom (CHCl3) layer is used as lipid fraction. Ribitol and nonadecanoic acid methyl ester were used for internal standards of polar and lipid fractions. Lipid fraction was methoximated with CHCl3/NaOH/MeOH. After washing by water, CHCl3 layer was dried over by N2 gas. Metabolites were trimethylsilylated with n-Heptane/Pyridine/MSTFA. GC conditions: Agilent 6890N GC (Agilent), Carrier gas: He, MS conditions: Pegasus III TOF-MS system (Leco).
Comment_of_details Instrument Type: GC-TOF-MS
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