SE218:/S04/M02

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Sample Set Information

ID SE218
Title Metabolome analysis of Carica papaya fruit
Description Investigation of Carica papaya fruit metabolites. 2 tissue (pulp and peel), 2 developmental stages (unripe and ripe) and 4 replicates data are examined.
Authors Yasuhide Hiraga 1,2, Takeshi Ara 2,3, Nao Sato 4, Nayumi Akimoto 2, Kenjiro Sugiyama 4, Hideyuki Suzuki 1,2, Kota Kera 5;1 HIRATA Corporation, 2 Kazusa DNA Research Institute, 3 Kyoto University, 4 Kogakuin University, 5 Tokyo University of Agriculture
Reference Hiraga Y. et al. (2021) Bioscience, Biotechnology, and Biochemistry (in press)
Comment


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Sample Information

ID S04
Title Carica papaya L. ripe fruit peel
Organism - Scientific Name Carica papaya L.
Organism - ID NCBI taxonomy:3649
Compound - ID
Compound - Source
Preparation Ripe and unripe papaya (Carica papaya L.) fruits were obtained from local markets in Okinawa prefecture.
Sample Preparation Details ID
Comment

Analytical Method Information

ID M02
Title LC-Orbitrap-MS, ESI Positive analysis
Method Details ID MS1
Sample Amount
Comment [MassBase ID] MDLC1_48657


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The raw (binary) and near-raw (text) files of this analysis are available at MassBase.

Analytical Method Details Information

ID MS1
Title LC-Orbitrap-MS ESI positive method
Instrument Ultimate 3000 RSLC (Thermo Fisher Scientific), Q Exactive MS (Thermo Fisher Scientific)
Instrument Type LC-Orbitrap-MS
Ionization ESI
Ion Mode Positive
Description The samples were cut into small pieces and then frozen in liquid N2 and resulting powder (100mg) are solved in 300uL 100% methanol solution and homogenized with zirconia beads in a TissueLyser II. The supernatant was filtered using a Mono-Spin C18 column and filtered through a 0.2 µm polyvinylidene difluoride (PVDF) membrane. 2µL of sample is injected into HPLC. HPLC conditions: Ultimate 3000 RSLC (Thermo Fisher Scientific Inc.), Column: InertSustain AQ-C18 (column size: 2.1 × 150 mm; particle size: 3.0 μm; GL Science Inc.), Solvent: A; 0.1% formic acid in water, B; 0.1% formic acid in acetonitrile, Gradient: 2% B for 0 to 3 min, 2 to 98% B for 3 to 30 min, 98% B for 30 to 35 min, and 2% B for 35 to 40 min. Column temp.: 40 degree C, Flow rate=0.2mL/min, PDA: 190-950 nm (2 nm step). Orbitrap-MS conditions: Q Exactive Mass Spectrometer (Thermo Fisher Scientific), ESI positive mode, spray voltage: 3.2 kV, capillary temperature: 300 degree C, Full mass scan condition (scan range: m/z 80–1200, resolution: 70000 at m/z 200, AGC target: 1e6), MS/MS conditoin: up to 10 data-dependent HCD MS2 scans (AGC target: 1e5, max IT: 50 ms, resolution: 17500 at m/z 200, isolation window: m/z 2.0, stepped (N)CE: 10/50/80, underfill ratio: 5%, and dynamic exclusion: 20 s).
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