SE2:/S01/M02/D02

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Sample Set Information

ID SE2
Title Lotus japonicus flower metabolite analysis
Description Investigation of Lotus japonicus flower metabolites. 4 replicates data are examined.
Authors Takeshi Ara, Ryosuke Sasaki, Mitsuo Enomoto, Nozomu Sakurai, Hideyuki Suzuki, Daisuke Shibata, Kazusa DNA Research Institute
Reference Direct Submittion
Comment version 5


Link icon database.png Link icon pgdbj.png

The web resources and information related to the species used in this study are available at Plant Genome Database Japan (PGDBj).

Sample Information

ID S01
Title Lotus japonicus MG-20 flower
Organism - Scientific Name Lotus japonicus
Organism - ID NCBI taxonomy:34305
Compound - ID
Compound - Source
Preparation Lotus japonicus MG-2 seeds are sown on pots filled by soil and are grown in natural conditions. After 2 months later, all flowers are harvested.
Sample Preparation Details ID
Comment [KomicMarket ID] KSBA_24

Analytical Method Information

ID M02
Title LC-FTICR-MS, ESI Positive analysis
Method Details ID MS1
Sample Amount 6.7 mg
Comment [MassBase ID] MDLC1_05775


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The raw (binary) and near-raw (text) files of this analysis are available at MassBase.

Analytical Method Details Information

ID MS1
Title LC-FT-ICR-MS ESI positive method 1
Instrument Agilent1100 HPLC (Agilent), LTQ-FT (Thermo Fisher Scientific)
Instrument Type LC-FTICR-MS
Ionization ESI
Ion Mode Positive
Description Harvested sample is frozen by liquid N2 and resulting powder (100mg) are solved in 300uL 80% methanol solution. 20uL sample is injected into HPLC after 0.2um membrane filter treatment. HPLC conditions: Agilent 1100 series (Agilent), Column: TSKgel-100V (4.6 x 250 mm, 5 micrometer; TOSOH), Solvent: A; 0.1% formic acid aq. B; ACN (addition 0.1% formic acid fc.), Gradient: (B);3 to 30% (0.0 to 25.0 min), 30 to 90% (25.0 to 40.0 min), 90% (40.0 to 45.0 min), 95% (45.1 to 50.0 min), 3% (50.1 to 57.0 min), Column temp.: 30 degree C, Flow rate=0.5mL/min, PDA: 200-650 nm (2 nm step). FT-ICR-MS conditions: Filter 1: FTMS + c norm !corona !pi res=50000 o(200.0-1500.0); 2: ITMS + c norm !corona !pi Dep MS/MS Most intense ion from (1).;3: ITMS + c norm o(200.0-1500.0)., Rejected mass=266.0000;294.0000;391.0000.
Comment_of_details

Data Analysis Information

ID D02
Title PowerGet data analysis for KomicMarket2
Data Analysis Details ID DS2
Recommended decimal places of m/z 6|ITMS 2
Comment


Link icon database2.png Link KM2.png

The peak data files written in TogoMD format are downloadable at the KomicMarket2 temporary web site.

Data Analysis Details Information

ID DS2
Title PowerGet analysis for detection of all peaks (B2)
Description Raw data files are converted to text file by MSGet software without cut off value and peaks are extracted from the text files by PowerFT with parameters (intensity cut off=5000, peak selection filter is default, peak shape is manually checked for peaks with intensity < 1000). The replicates data are aligned by PowerMatch with blank data. The alignment is manually edited. Assigned peaks observed in multiple replicate samples are selected for annotation process.
Comment_of_details
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