SE37:/S19/M1

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Sample Set Information

ID SE37
Title Medicago truncatula shoot metabolite analysis using stable isotopes
Description Metabolites in the Medicago truncatula shoot are investigated by using stable isotope labelings.

This analysis was performed to estimate the elemental composition with high reliability using the number of stable isotopes incorporated in the compound as an index and to add information to discuss the biosynthetic pathway. The Medicago seedlings were labeled with 13C, 15N, 18O, and 34S, respectively in the same condition. Following peak picking by using PowerFT (S01 to S16), the metabolite list was prepared from unlabeled data and blank data (S17). The isotopic peaks were searched and listed using ShiftedIonsFinder (S18). Moreover, the flavonoid-like peaks were also searched using ShiftedIonsFinder (S19).

Authors AUTHERS; Kota Kera1, Yoshiyuki Ogata2, Takeshi Ara1, Yoshiki Nagashima1, Norimoto Shimada1,3, Nozomu Sakurai1. Daisuke Shibata1 and Hideyuki Suzuki1*

AFFILIATION; 1 Kazusa DNA Research Institute, Kisarazu, Chiba 292-0818, Japan; 2 Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Osaka 599-8531, Japan; 3 Present Address, Tokiwa Phytochemical Co.,Ltd. 158 Kinoko, Sakura, Chiba 285-0801, Japan

Reference ShiftedIonsFinder: A standalone Java tool for finding peaks with specified mass differences by comparing the mass spectra of isotope-labeled and unlabeled data sets. Plant Biotechnology (2014) 3: 269-274

Pathway-specific metabolome analysis with 18O2-labeled Medicago truncatula via a mass spectrometry-based approach. Metabolomics (2018) 14:71

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Sample Information

ID S19
Title PSEUDO: Unlabeled and labeled Medicago samples for flavonoid annotation using ShiftedIonsFinder
Organism - Scientific Name
Organism - ID
Compound - ID
Compound - Source
Preparation
Sample Preparation Details ID
Comment This is a pseudo metadata prepared for integrated analyses of multiple datasets.

The Medicago samples (S01, S02, and S03) and a blank sample (S16) are included.

Analytical Method Information

ID M1
Title
Method Details ID
Sample Amount
Comment This is a pseudo metadata prepared for integrated analyses of multiple datasets.

The raw data obtained from LC-Orbitrap MS analysis for Medicago samples (S01_M1, S02_M1, and S03_M1) and for a blank sample (S16_M1 to S16_M3) are used. Import file name is "unlabeling"


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