SE39:/S02/M03
From Metabolonote
lower pageD01
Sample Set Information
| ID | SE39 |
|---|---|
| Title | Comparison of flower metabolites among Cosmos bipinnatus varieties |
| Description | Investigation of Cosmos bipinnatus Flower metabolites. 4 colors and 3 replicates data are examined. |
| Authors | Takeshi Ara, Daisuke Nakajima, Mitsuo Enomoto, Nozomu Sakurai, Hideyuki Suzuki, Koei Okazaki, Daisuke Shibata, Kazusa DNA Research Institute |
| Reference | Direct Submittion |
| Comment |
Sample Information
| ID | S02 |
|---|---|
| Title | Cosmos bipinnatus Flower (Pink color) |
| Organism - Scientific Name | Cosmos bipinnatus |
| Organism - ID | NCBI taxonomy:51277 |
| Compound - ID | |
| Compound - Source | |
| Preparation | Cosmos bipinnatus flowers are harvested at Kisarazu, Chiba, Japan. |
| Sample Preparation Details ID | |
| Comment |
Analytical Method Information
| ID | M03 |
|---|---|
| Title | LC-FTICR-MS, ESI Positive analysis |
| Method Details ID | MS1 |
| Sample Amount | 6.7mg |
| Comment | [MassBase ID] MDLC1_19251 |
The raw (binary) and near-raw (text) files of this analysis are available at MassBase.
Analytical Method Details Information
| ID | MS1 |
|---|---|
| Title | LC-FT-ICR-MS ESI positive method |
| Instrument | Agilent1100 HPLC (Agilent), LTQ-FT (Thermo Fisher Scientific) |
| Instrument Type | LC-FTICR-MS |
| Ionization | ESI |
| Ion Mode | Positive |
| Description | Sample is frozen by liquid N2 and resulting powder (100mg) are solved in 300uL 80% methanol solution. 20uL sample is injected into HPLC after 0.2um membrane filter treatment. HPLC conditions: Agilent 1100 series (Agilent), Column: TSKgel-100Z (4.6 x 250 mm, 5 micrometer; TOSOH), Solvent: A; 0.1% formic acid aq. B; ACN (addition 0.1% formic acid fc.), Gradient: (B);3 to 97% (0.0 to 45.0 min), 97% (45.1 to 50.0 min), 3% (50.1 to 57.0 min), Column temp.: 40 degree C, Flow rate=0.5mL/min, PDA: 200-650 nm (2 nm step). FT-ICR-MS conditions: Filter 1: FTMS + c norm !corona !pi res=100000 o(100.0-1500.0); 2: ITMS + c norm !corona !pi Dep MS/MS Most intense ion from (1), 3: ITMS + c norm !corona !pi Dep MS/MS 2nd most intense ion from (1), 4: ITMS + c norm !corona !pi Dep MS/MS 3rd most intense ion from (1), 5: ITMS + c norm !corona !pi Dep MS/MS 4th most intense ion from (1), 6: ITMS + c norm !corona !pi Dep MS/MS 5th most intense ion from (1). Reject Mass List:235.2000, 236.2000, 376.0000, 377.0000, 609.3000, 610.3000, 810.4000, 811.4000, 1123.7000, 1124.7000. |
| Comment_of_details |
