SE3:/S04/M03

From Metabolonote
jump-to-nav Jump to: navigation, search

Sample Set Information

ID SE3
Title Comparison of fruit metabolites among tomato varieties 1
Description Investigation of Solanum lycopersicum fruit metabolites. 3 caltivars and an original species, 3 replicates data are examined.
Authors Naoki Yamamoto 1, Takeshi Ara 1, Tatsuya Suzuki 2, Taneaki Tsugane 2, Sayaka Shinpo 1, Yoshihiko Morishita 1, Ryosuke Sasaki 1, Mitsuo Enomoto 1, Nozomu Sakurai 1, Hideyuki Suzuki 1, Daisuke Shibata 1, 1: Kazusa DNA Research Institute, 2: Chiba Prefectural Agriculture Research Center
Reference Yamamoto N et al. (2012) Plant Biotechnology 29: 109-113
Comment version 5


Link icon database.png Link icon pgdbj.png

The web resources and information related to the species used in this study are available at Plant Genome Database Japan (PGDBj).

Sample Information

ID S04
Title Solanum peruvianum LA3858 Fruit
Organism - Scientific Name Solanum peruvianum
Organism - ID NCBI taxonomy:4082
Compound - ID
Compound - Source
Preparation Solanum peruvianum LA3858 Fruit are grown at agricultural field under normal film in natural conditions.
Sample Preparation Details ID
Comment [KomicMarket ID] KSBA_18

Analytical Method Information

ID M03
Title LC-FTICR-MS, ESI Positive analysis
Method Details ID MS1
Sample Amount 6.7mg
Comment [MassBase ID] MDLC1_25538


Link icon database.png Link icon massbase.png

The raw (binary) and near-raw (text) files of this analysis are available at MassBase.

Analytical Method Details Information

ID MS1
Title LC-FT-ICR-MS ESI positive method 1
Instrument Agilent1100 HPLC (Agilent), LTQ-FT (Thermo Fisher Scientific)
Instrument Type LC-FTICR-MS
Ionization ESI
Ion Mode Positive
Description Harvested sample is frozen by liquid N2 and resulting powder (100mg) are solved in 300uL 80% methanol solution. 20uL sample is injected into HPLC after 0.2um membrane filter treatment. HPLC conditions: Agilent 1100 series (Agilent), Column: TSKgel-100V (4.6 x 250 mm, 5 micrometer; TOSOH), Solvent: A; 0.1% formic acid aq. B; ACN (addition 0.1% formic acid fc.), Gradient: (B);3 to 50% (0.0 to 20.0 min), 50 to 90% (20.0 to 40.0 min), 90% (40.0 to 45.0 min), 95% (45.1 to 50.0 min), 3% (50.1 to 57.0 min), Column temp.: 40 degree C, Flow rate=0.5mL/min, PDA: 200-650 nm (2 nm step). FT-ICR-MS conditions: Filter 1: FTMS + c norm !corona !pi res=100000 o(100.0-1500.0); 2: ITMS + c norm !corona !pi Dep MS/MS Most intense ion from (1); 3: ITMS + c norm !corona !pi Dep MS/MS 2nd most intense ion from (1); 4: ITMS + c norm !corona !pi Dep MS/MS 3rd most intense ion from (1); 5: ITMS + c norm !corona !pi Dep MS/MS 4th most intense ion from (1); 6: ITMS + c norm !corona !pi Dep MS/MS 5th most intense ion from (1)., Rejected mass=294.2600, 376.0400, 609.2800, 810.4200, 1123.6800
Comment_of_details


link=SE14_MS1

The data can be compared with the data obtained by the method SE14_MS1

Personal tools
View and Edit Metadata
Variants
Views
Actions