SE40:/S26/M03/D01

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Sample Set Information

ID SE40
Title Comparison of fruit metabolites among tomato cultivars
Description Investigation of Tomato fruits metabolites. 28 cultivers and 3 replicates data are examined.
Authors Takeshi Ara 1, Shingo Takahashi 1,3, Naoko Waki 1,3, Nozomu Sakurai 2, Haruya Takahashi 1, Shinsuke Mohri 1, Kunimi Suda 2, Keishi Ozawa 2, Takisawa Rihito 4, Akira Kitajima 4, Yasuki Matsumura 1, Teruo Kawada 1, Daisuke Shibata 1, 2, 1:Graduate School of Agriculture, Kyoto University, Japan, 2:Kazusa DNA Research Institute, Kazusa Kamatari 2-6-7, Kisarazu, Chiba 292-0818, Japan, 3:Kagome Co., Ltd., Japan, 4:Experimental Farm of Kyoto University, Japan
Reference Ara T. et al. (2021) Plant Direct 5(4) e00318
Comment version 1


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TOMATOMET: http://metabolites.in/tomato-fruits/

PGDBj: http://pgdbj.jp/plantdb/plantinfo.html?ln=en&cmd=entry&ppid=t4081

Sample Information

ID S26
Title Solanum lycopersicum Sicilian Rouge fruit
Organism - Scientific Name Solanum lycopersicum
Organism - ID NCBI taxonomy:4081
Compound - ID
Compound - Source
Preparation Solanum lycopersicum Sicilian Rouge were purchased at a local market of Kyoto city, (Kyoto, Japan) (November, 2015).
Sample Preparation Details ID
Comment Kyoto-san.

Analytical Method Information

ID M03
Title LC-Orbitrap-MS, ESI Positive analysis
Method Details ID MS2
Sample Amount
Comment [MassBase ID] MDLC1_47209


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The raw (binary) and near-raw (text) files of this analysis are available at MassBase.

Analytical Method Details Information

ID MS2
Title LC-Orbitrap-MS ESI positive method 2
Instrument Nexera HPLC (Shimadzu), LTQ-Orbitrap Velos Pro (Thermo Fisher Scientific)
Instrument Type LC-Orbitrap-MS
Ionization ESI
Ion Mode Positive
Description Harvested sample is frozen by liquid N2 and resulting powder (100mg) are solved in 300uL 80% methanol solution. 5uL sample is injected into HPLC after 0.2um membrane filter treatment. HPLC conditions: Shimadzu Nexera (Shimadzu), Column: TSKgel ODS-100V (4.6 x 250 mm, 5 micrometer; TOSOH), Solvent: A; 0.1% formic acid aq. B; ACN (addition 0.1% formic acid fc.), Gradient: (B) 0 to 3% for 15 min, 3 to 97% for 20 min, 97 to 3% for 0.1 min, and 3% for 25 min., Column temp.: 40 degree C, Flow rate=0.4mL/min, PDA: 190-800 nm (2 nm step). Orbitrap-MS conditions: Filter 1: FTMS + c norm !corona !pi res=60000 o(100.0-1500.0); 2: ITMS + c norm !corona !pi Dep MS/MS Most intense ion from (1); 3: ITMS + c norm !corona !pi Dep MS/MS 2nd most intense ion from (1); 4: ITMS + c norm !corona !pi Dep MS/MS 3rd most intense ion from (1); 5: ITMS + c norm !corona !pi Dep MS/MS 4th most intense ion from (1).
Comment_of_details

Data Analysis Information

ID D01
Title PowerGet data analysis
Data Analysis Details ID DS1
Recommended decimal places of m/z 6|ITMS 2
Comment


Data Analysis Details Information

ID DS1
Title PowerGet analysis for detection of all peaks
Description Raw data files are converted to text file by MSGet software without cut off value and peaks are extracted from the text files by PowerFT with parameters (intensity cut off=20000, margin of ion grouping in peak detection=5, peak selection filter is default, intensity cut off in peak assignment=1000). The replicates data are aligned by PowerMatch with blank data. The alignment is manually edited. Assigned peaks observed in multiple replicate samples are selected for annotation process.
Comment_of_details
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