SE42:/DS1

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Sample Set Information

ID SE42
Title Metabolic profiling of flavonoids in Lotus japonicus
Description Metabolic profiling of flavonoids in leaf, stem, flower of Lotus japonicus Miyakojima MG-20 and Gifu B-129.
Authors Hideyuki Suzuki 1, Ryosuke Sasaki 1, Yoshiyuki Ogata 1, Yukiko Nakamura 2 4, Nozomu Sakurai 1, Mariko Kitajima 3, Hiromitsu Takayama 3, Shigehiko Kanaya 2, Koh Aoki 1, Daisuke Shibata 1, Kazuki Saito 3, 1: Kazusa DNA Research Institute, 2: Nara Institute of Science and Technology, 3: hiba University, 4: Ehime Women’s College
Reference Phytochemistry, 2008, 69(1), 99-111
Comment Seed coats of L. japonicus were scratched by glass paper and fed water for 1 d. The seedlings were transferred to a mixture of vermiculite and a commercial soil, Powersoil (mix ratio 1.3–1, Kureha Chemical Ind., Tokyo, Japan, and Kanto Hiryou Ind., Saitama, Japan), and grown for 90–120 days (from March to June) in 2005, in a greenhouse under natural sunlight. From 7-day-old plants, cotyledons and hypocotyls were collected separately and served as leaf and stem samples, respectively. From 14-, 21-, 30-, 60, and 90-day-old plants, leaves and stems were collected. The tissues for one experimental replicate were collected from 12 independent plants for 7-, 14-, and 21-day-old stages, or from one independent plant for 30-, 60-, and 90-day-old stages.


Link icon database.png Link icon pgdbj.png

The web resources and information related to the species used in this study are available at Plant Genome Database Japan (PGDBj).

http://pgdbj.jp/plantdb/plantinfo.html?ln=en&cmd=entry&ppid=t34305

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Data Analysis Details Information

ID DS1
Title PowerGet analysis for annotation of peaks
Description Raw data files are converted to text file by MSGet software without cut off value and peaks are extracted from the text files by PowerFT. The replicates data are aligned by PowerMatch with blank data. The alignment is manually edited. Assigned peaks observed in multiple replicate samples are selected for annotation process.
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