SE43:/S03/M02
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Sample Set Information
| ID | SE43 |
|---|---|
| Title | Comaparaive metabolite analysis of three Allium species |
| Description | Investigation of Allium species leaf metabolites. 3 species, 4 replicates data are examined. |
| Authors | Takeshi Ara 1,2, Kunihiro Suda 1, Masayuki Amagai 3,4,5, Kiyoshi Namai 3,6, Hideyuki Suzuki 1,7, Nozomu Sakurai 1,8, Daisuke Shibata 1,4, 1:Kazusa DNA Research Institute, 2:Research Institute for Sustainable Humanosphere, Kyoto University, 3:Tochigi Agricultural Experimentation Station, 4:Department of Biological Sciences, Kisarazu Campus of Tohoku University, 5:Ansoku Agriculture Promotion Office, 6:Tochigi Agricultural College, 7:Hirata Corporation, 8:Department of Informatics, National Institute of Genetics. |
| Reference | Ara T. et al. (2020) Plant Biotechnology 37(3), 383-387. |
| Comment |
Sample Information
| ID | S03 |
|---|---|
| Title | Allium fistulosum L. Nissatonegi |
| Organism - Scientific Name | Allium fistulosum |
| Organism - ID | NCBI taxonomy:35875 |
| Compound - ID | |
| Compound - Source | |
| Preparation | Mature leaves were harvested (four biological replicates per cultivar), immediately frozen in liquid nitrogen, and then kept at −80 degree C. |
| Sample Preparation Details ID | |
| Comment |
Analytical Method Information
| ID | M02 |
|---|---|
| Title | LC-FTICR-MS, ESI Positive analysis |
| Method Details ID | MS1 |
| Sample Amount | 6.7mg |
| Comment | [MassBase ID] MDLC1_32616 |
The raw (binary) and near-raw (text) files of this analysis are available at MassBase.
Analytical Method Details Information
| ID | MS1 |
|---|---|
| Title | LC-FT-ICR-MS ESI positive method 1 |
| Instrument | Agilent1100 HPLC (Agilent), LTQ-FT (Thermo Fisher Scientific) |
| Instrument Type | LC-FTICR-MS |
| Ionization | ESI |
| Ion Mode | Positive |
| Description | Harvested sample is frozen by liquid N2 and resulting powder (100mg) are solved in 300uL 80% methanol solution. 20uL sample is injected into HPLC after 0.2um membrane filter treatment. HPLC conditions: Agilent 1100 series (Agilent), Column: TSKgel-100V (4.6 x 250 mm, 5 micrometer; TOSOH), Solvent: A; 0.1% formic acid aq. B; ACN (addition 0.1% formic acid fc.), Gradient: (B);3 to 97% (0.0 to 45.0 min), 97% (45.0 to 50.0 min), 97% to 3% (50 to 50.1 min), 3% (50.1 to 57.0 min), Column temp.: 40 degree C, Flow rate=0.5mL/min, PDA: 200-650 nm (2 nm step). FT-ICR-MS conditions: Filter 1: FTMS + c norm !corona !pi res=100000 o(100.0-1500.0); 2: ITMS + c norm !corona !pi Dep MS/MS Most intense ion from (1); 3: ITMS + c norm !corona !pi Dep MS/MS 2nd most intense ion from (1); 4: ITMS + c norm !corona !pi Dep MS/MS 3rd most intense ion from (1); 5: ITMS + c norm !corona !pi Dep MS/MS 4th most intense ion from (1); 6: ITMS + c norm !corona !pi Dep MS/MS 5th most intense ion from (1)., Rejected mass=224.0100, 235.1800, 241.0700, 266.2300, 282.1000, 315.0900, 376.0400, 378.0400, 389.1100, 463.1300, 480.1600, 534.0500, 537.1500, 540.6000, 554.1800, 609.2800, 628.2000, 702.2200, 726.3600, 810.4200, 1101.7000, 1118.7200, 1123.6800 |
| Comment_of_details |
