SE44:/DS1
From Metabolonote
Sample Set Information
| ID | SE44 |
|---|---|
| Title | Physcomitrella patens metabolite analysis using stable isotopes |
| Description | Metabolites in the Physcomitrella patens are investigated by using stable isotope labelings. This analysis was performed to estimate the elemental composition with high reliability using the number of stable isotopes incorporated in the compound as an index and to add information to discuss the biosynthetic pathway. The Physcomitrella patens were labeled with 13C, 15N, 18O, and 34S, respectively in the same condition. Following peak picking by using PowerGet, the metabolite list was prepared from unlabeled data and blank data. The isotopic peaks were searched and listed using ShiftedIonsFinder. Moreover, the flavonoid-like peaks were also searched using ShiftedIonsFinder. |
| Authors | Yasuhide Hiraga 1,2, Takeshi Ara 1, Yoshiki Nagashima 1, Nozomu Sakurai 1, Hideyuki Suzuki 1,2, Kota Kera 1,3; 1:Kazusa DNA Research Institute, Japan, 2:Hirata Corporation, Japan, 3:Faculty of Applied Bioscience, Tokyo University of Agriculture, Japan |
| Reference | Hiraga Y, et al, (2020) Plant Biotechnology, 37(3), 377-381. |
| Comment |
Data Analysis Details Information
| ID | DS1 |
|---|---|
| Title | PowerGet analysis for detection of all peaks |
| Description | Raw data files are converted to text file by MSGet software without cut off value and peaks are extracted from the text files by PowerFT with parameters (intensity cut off=20000, margin of ion grouping in peak detection=5, peak selection filter is default, intensity cut off in peak assignment=1000). The replicates data are aligned by PowerMatch with blank data. The alignment is manually edited. Assigned peaks observed in multiple replicate samples are selected for annotation process. |
| Comment_of_details |
