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Sample Set Information

Title Covering chemical diversity of genetically-modified tomatoes using metabolomics for objective substantial equivalence assessment
Description We propose using multiple analytical platforms for the direct acquisition of an interpretable data set of estimable chemical diversity. As an example, we report an application of our multi-platform approach that assesses the substantial equivalence of tomatoes over-expressing the taste-modifying protein miraculin. In combination, the chosen platforms detected compounds that represent 86% of the estimated chemical diversity of the metabolites listed in the LycoCyc database. Following a proof-of-safety approach, we show that w92% had an acceptable range of variation while simultaneously indicating a reproducible transformation-related metabolic signature. We conclude that multi-platform metabolomics is an approach that is both sensitive and robust and that it constitutes a good starting point for characterizing genetically modified organisms.
Authors Miyako Kusano, Henning Redestig, Tadayoshi Hirai, Akira Oikawa, Fumio Matsuda, Atsushi Fukushima, Masanori Arita, Shin Watanabe, Megumu Yano, Kyoko Hiwasa-Tanase, Hiroshi Ezura, Kazuki Saito
Reference Kusano M et al. (2011) PLOS ONE 6: e16989

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The raw data files are available at DROP Met web site in PRIMe database of RIKEN.

Sample Information

ID S01
Title Tomato
Organism - Scientific Name Solanum lycopersicum
Organism - ID NCBI taxonomy:4081
Compound - ID
Compound - Source
Preparation Solanum lycopersicum, L. cv. Moneymaker, Aichi-first, Ailsa Craig, MicroTom, M82, and Rutgers.

<Biosource species>
The two transgenic lines overexpressing milaculin gene (moneymaker background) were also used.



<Organ specification>

Green and red fruits. A three grade color scale (green, orange, and red) was employed to evaluate tomato color.

Sample Preparation Details ID SS01

Sample Preparation Details Information

Title Growth condition and Sampling
Description <Growth condition>

Seedlings of Solanum lycopersicum were potted in 1/2000 a Wagner pot containing compost soil (Kureha, Tokyo, Japan) for the soil experiment. Seeds were sown in 5 cm × 5 cm × 5 cm (height × length × width) rockwool cubes and grown in a hydroponics system (565 mg l-1 NO- 3 , 15.7 mg l-1 NH+4 , 202.2 mg l-1 PO- 3 , 218.4 mg l-1 K+, 19.9 mg l-1 Mg+2 , 95.0 mg l-1 Ca+2 and micronutrients) in an environmentally controlled growth room at 25 °C/20 °C (light/dark) and 600 ppm CO2 concentration with a light/dark cycle of 16 h/8 h for the hydroponic culture (HC) experiment. Seedlings were placed in a netted-greenhouse located at the Gene Research Center in University of Tsukuba

<Sampling and sampling date>

The fruits were harvested in spring (a pilot and HC experiments) and late summer (the soil experiment) in 2006, 2008, and 2009.

<Metabolism quenching method>

All samples were frozen within 30 s after sampling in liquid nitrogen. The frozen samples were lyophilized

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