SE59:/DS01

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Sample Set Information

ID	SE59
Title	Alternation of flavonoid accumulation under drought stress in Arabidopsis thaliana
Description	We reported that flavonoids, a class of specialized metabolites, including flavonols and anthocyanins with strong radical scavenging activity contributed to the mitigation of oxidative and drought stress in Arabidopsis thaliana (Arabidopsis). However, the behavior of flavonoids during drought stress is still not well documented. Herein we investigated the time-series alternation of flavonoids in the aerial part of Arabidopsis (wild type, Col-0) during drought stress by LC-QTOF-MS. The drastic alternation of 5 flavonols and 5 anthocyanins was revealed together with changes in marker metabolites of drought stress, e.g., proline, raffinose, and galactinol. These findings indicate that flavonols and anthocyanins can mitigate drought stress.
Authors	Ryo Nakabayashi, Tetsuya Mori, and Kazuki Saito
Reference	Nakabayashi R et al. (2014) Plant Signaling & Behavior 9: e29518
Comment

ID	DS01
Title	Data analysis
Description	<Untargeted metabolomic analysis of LC-QTOF-MS> The untargeted metabolomic analysis using LC-QTOF-MS and the metabolome data processing of alignment, de-isotoping, noise cutting, and normalization was conducted as previously described. <Statistical analysis> Principal component analysis (PCA) using the processed data consisting of 4093 metabolite peaks was performed with the SIMCA-P 11.5 software, and HCA using the data of the assigned flavonoids was performed by MeV 4.8 (http://mev.tm4.org/). <Data upload> Metabolomic data are available on the website DROP Met < http://prime.psc.riken.jp/?action=drop_index>.
Comment_of_details