SE59:/MS01
Sample Set Information
| ID | SE59 |
|---|---|
| Title | Alternation of flavonoid accumulation under drought stress in Arabidopsis thaliana |
| Description | We reported that flavonoids, a class of specialized metabolites, including flavonols and anthocyanins with strong radical scavenging activity contributed to the mitigation of oxidative and drought stress in Arabidopsis thaliana (Arabidopsis). However, the behavior of flavonoids during drought stress is still not well documented. Herein we investigated the time-series alternation of flavonoids in the aerial part of Arabidopsis (wild type, Col-0) during drought stress by LC-QTOF-MS. The drastic alternation of 5 flavonols and 5 anthocyanins was revealed together with changes in marker metabolites of drought stress, e.g., proline, raffinose, and galactinol. These findings indicate that flavonols and anthocyanins can mitigate drought stress. |
| Authors | Ryo Nakabayashi, Tetsuya Mori, and Kazuki Saito |
| Reference | Nakabayashi R et al. (2014) Plant Signaling & Behavior 9: e29518 |
| Comment |
The raw data files are available at DROP Met web site in PRIMe database of RIKEN.
Analytical Method Details Information
| ID | MS01 |
|---|---|
| Title | Untargeted metabolomic analysis of LC-QTOF-MS |
| Instrument | LC, Waters Acquity UPLC system; MS, Waters Xevo G2 Q-Tof |
| Instrument Type | UPLC-QTOF-MS |
| Ionization | ESI |
| Ion Mode | Positive |
| Description | Tissue processing method: The lyophilized materials were ground using a mixer mill (MM300, Retsch) with zirconia beads for 2 min at 18 Hz and 4 °C.
Storage condition prior to extraction or further processing: The lyophilized powder materials were stored at −80 °C. Extraction method: The freeze-dried samples were extracted with 50 ml of 80% MeOH containing 2.5 mM lidocaine per mg dry weight using a mixer mill (MM300, Retsch) with zirconia beads for 10 min at 20 Hz and 4 °C. Extract cleanup and/or Additional manipulation: After centrifugation for 10 min, the supernatant was filtered using an HLB mElution plate (Waters). Extract storage and/or relocate: not storaged Chromatography instrument description: LC, Waters Acquity UPLC system Separation column and pre/guard column: column, Acquity BEH C18 (1.7 μm, 2.1 mm x 100 mm, Waters) Separation parameter: solvent system, solvent A (water with 0.1% formic acid) and solvent B (acetonitrile with 0.1% formic acid) Instrument description: Waters Xevo G2 Q-Tof Sample introduction and delivery: From LC Ionization source: ESI positive. column temperature, 40 °C; MS detection: capillary voltage, +3.0 keV, cone voltage, 25.0 V, source temperature, 120 °C, desolvation temperature, 450 °C, cone gas flow, 50 l/h; desolvation gas flow, 800 l/h; collision energy, 6 V; MS/MS detection: collision energy, rampled from 10 to 50V Mass analyzer description and acquisition mode: quadrupole-time-of-flight Data acquisition parameters: MS: mass range, m/z 100‒1500; scan duration, 0.1 sec; interscan delay, 0.014 sec; data acquisition, centroid mode. MS/MS: m/z 50–1500; scan duration, 0.02 sec; inter-scan delay, 0.014 sec; data acquisition, centroid mode; MS/MS spectra of the top 10 ions (> 1000 counts) in an MS scan were automatically obtained. |
| Comment_of_details |
