SE59:/S01

Sample Set Information

ID	SE59
Title	Alternation of flavonoid accumulation under drought stress in Arabidopsis thaliana
Description	We reported that flavonoids, a class of specialized metabolites, including flavonols and anthocyanins with strong radical scavenging activity contributed to the mitigation of oxidative and drought stress in Arabidopsis thaliana (Arabidopsis). However, the behavior of flavonoids during drought stress is still not well documented. Herein we investigated the time-series alternation of flavonoids in the aerial part of Arabidopsis (wild type, Col-0) during drought stress by LC-QTOF-MS. The drastic alternation of 5 flavonols and 5 anthocyanins was revealed together with changes in marker metabolites of drought stress, e.g., proline, raffinose, and galactinol. These findings indicate that flavonols and anthocyanins can mitigate drought stress.
Authors	Ryo Nakabayashi, Tetsuya Mori, and Kazuki Saito
Reference	Nakabayashi R et al. (2014) Plant Signaling & Behavior 9: e29518
Comment

PubMed

The raw data files are available at DROP Met web site in PRIMe database of RIKEN.

Sample Information

ID	S01
Title	Arabidopsis
Organism - Scientific Name	Arabidopsis thaliana
Organism - ID	NCBI taxonomy:3702
Compound - ID
Compound - Source
Preparation	Treatment dose or intensity levels: The plants vertically grown in GM plates were incubated in 24-well plates (TPP, http://www.tpp.ch/index.php) and each plant was put in a well with 3 ml water. Treatment time, time intervals and duration before harvest: 0 day Harvest date, time: no information Plant growth stage: 3 weeks (GM plate) + 0 day (treatment) Metabolism quenching method: Freezing with liquid N2 Harvest method: After removing root, the samples were immediately frozen with liquid nitrogen and lyophilized at −55 °C. Sample strage: The lyophilized materials were stored at −80 °C. Reprecate sampling and analyses: n=3 Tissue harvesting method: Time from tissue resection to liquid N2 freezing was one minute. Tissues were pooled in tube.
Sample Preparation Details ID	SS01
Comment

Sample Preparation Details Information

ID	SS01
Title	Drought stress
Description	Three-week-old plants vertically grown on GM plates were incubated in 24-well plates (TPP, http://www.tpp.ch/index.php). Each plant was put in a well with 3 ml water. Three-week-old plants were exposed to drought stress by stopping watering.The aerial samples were harvested at the time points of DAD 0, 3, and 5.
Comment_of_details	DAD, days after drought.