Sample Set Information
|Title||LC-MS analysis of parsley|
|Description|| Comprehensive analysis of metabolites in parsley (Petroselium crispum, Paramount) was performed by several combination of sample preparation and analytical conditions.
Parsley was hydroponically grown in a greenhouse (S11-S13) or a cultivation shelf (S21-S26) using control medium and stable isotope (15N or 34S)-containing medium.
|Authors||Sakurai N, Suda K (Kazusa DNA Research Insitute)|
|Reference||Akimoto N, Ara T, Nakajima D, Suda K, Ikeda C, Takahashi S, Muneto R, Yamada M, Suzuki H, Shibata D and Sakurai N (2017) FlavonoidSearch: A system for comprehensive flavonoid annotation by mass spectrometry. Scientific Reports 7: 1243|
|Title||Shoot of cultivation shelf grown parsley (15N labeled)|
|Organism - Scientific Name||Petroselinum crispum, Paramount|
|Organism - ID||NCBI taxonomy:4043|
|Compound - ID|
|Compound - Source|
|Preparation||Hydroponically grown in a cultivation shelf. Nitrogen in the culture medium was replaced with 15N. Shoot was harvested.|
|Sample Preparation Details ID||SS2|
Sample Preparation Details Information
|Title||Cultivation in a cultivation shelf|
|Description||The parsley seeds (Kaneko Seeds Co. LTD., KS100-542, Gunma, Japan) were germinated on a watered filter paper (Whatman 1002-150, GE Healthcare Japan) in a petri dish, and seedlings were grown 14 days in a cultivation shelf under fluorescent lamps (Biolux-A, NEC Corp., Tokyo, Japan) in 16 hr light / 8 hr dark regime at 25 ºC. The seedlings were transplanted on vermiculite (10865595 SK Agri K.K., Gunma, Japan), and grown 164 days under the same condition fertilizing with a culture medium containing 3 mM KNO3, 1 mM Ca(NO3)2, 0.5 mM MgSO4, 0.5 mM NH4H2PO4, 50 µM Fe-EDTA, 49 µM H3BO3, 9 µM MnSO4, 0.8 µM ZnSO4, 0.3 µM CuSO4, and 0.1 µM Na2MoO4. Shoot was harvested, ground to powder in liquid nitrogen by mortar and pestle, and stored at -80 ºC until use.|