SE8:/S01/M03/D03

From Metabolonote
jump-to-nav Jump to: navigation, search

Sample Set Information

ID SE8
Title Grifola frondosa metabolite analysis
Description Investigation of Grifola frondosa metabolites. 4 replicates data are examined.
Authors Takeshi Ara, Daisuke Nakajima, Mitsuo Enomoto, Nozomu Sakurai, Hideyuki Suzuki, Koei Okazaki, Daisuke Shibata, Kazusa DNA Research Institute
Reference Direct Submittion
Comment version 1

Sample Information

ID S01
Title Grifola frondosa
Organism - Scientific Name Grifola frondosa
Organism - ID NCBI taxonomy:5627
Compound - ID
Compound - Source
Preparation The Maitake mushroom samples are purchased at the local market.
Sample Preparation Details ID
Comment [KomicMarket ID] KSBA_29

Analytical Method Information

ID M03
Title LC-FTICR-MS, ESI Positive analysis
Method Details ID MS3
Sample Amount 6.7mg
Comment [MassBase ID] MDLC1_10303


Link icon database.png Link icon massbase.png

The raw (binary) and near-raw (text) files of this analysis are available at MassBase.

Analytical Method Details Information

ID MS3
Title LC-FT-ICR-MS ESI positive method 3
Instrument Agilent1100 HPLC (Agilent), LTQ-FT (Thermo Fisher Scientific)
Instrument Type LC-FTICR-MS
Ionization ESI
Ion Mode Positive
Description Sample is frozen by liquid N2 and resulting powder (100mg) are solved in 300uL 80% methanol solution. 20uL sample is injected into HPLC after 0.2um membrane filter treatment. HPLC conditions: Agilent 1100 series (Agilent), Column: TSKgel-100Z (4.6 x 250 mm, 5 micrometer; TOSOH), Solvent: A; 0.1% formic acid aq. B; ACN (addition 0.1% formic acid fc.), Gradient: (B);3 to 30% (0.0 to 25.0 min), 30 to 90% (25.1 to 40.0 min), 95% (45.1 to 50.0 min), 3% (50.1 to 57.0 min), Column temp.: 30 degree C, Flow rate=0.5mL/min, PDA: 200-650 nm (2 nm step). FT-ICR-MS conditions: Filter 1: FTMS + c norm !corona !pi res=100000 o(100.0-1600.0); 2: ITMS + c norm !corona !pi Dep MS/MS Most intense ion from (1); 3: ITMS + c norm Dep MS/MS 2nd most intense ion from (1); 4: ITMS + c norm Dep MS/MS 3rd most intense ion from (1); 5: ITMS + c norm Dep MS/MS 4th most intense ion from (1); 6: ITMS + c norm Dep MS/MS 5th most intense ion from (1), Reject Mass List:198.1800 224.1900 249.2100 266.2300 271.1900 282.2800 294.2600 427.3800 440.2800 472.4400 522.2800 522.3600 554.1800 662.4800 742.5400 756.5500 758.5700 782.5700 783.5700 784.5900 804.5500 950.8100 955.7500.
Comment_of_details

Data Analysis Information

ID D03
Title PowerGet data analysis for KomicMarket
Data Analysis Details ID DS3
Recommended decimal places of m/z 6|ITMS 2
Comment [KomicMarket ID] KSBA_29


Link icon database.png Komicmarket logo.png

The annotation data and quantitative data of the metabolite peaks in this sample are available at KomicMarket.

Data Analysis Details Information

ID DS3
Title PowerGet analysis for detection of all peaks (C3)
Description Raw data files are converted to text file by MSGet software without cut off value and peaks are extracted from the text files by PowerFT with parameters (intensity cut off=5000, peak selection filter is default, intensity cut off in peak assignment=1000).
Comment_of_details
Personal tools
View and Edit Metadata
Variants
Views
Actions