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Sample Set Information

Title Analysis of Lotus japonicus
Description Leaves of Lotus japonicus lines (MG-20 and B-129) were analyzed by LC-FT-ICR-MS. Two Arabidopsis thaliana samples were used as references.
Authors Takeshi Ara, Hideyuki Suzuki, Daisuke Shibata, Nozomu Sakurai, Ryosuke Sasaki, Makoto Hirosawa, Lab. Genome Biotech, Kazusa DNA Research Institute, Japan; Center for Development of Biotech Industries, Kazusa DNA Research Institute, Japan
Reference Direct submission
Comment NS deposited the metadata, most of which were retrieved from MassBase. For more detailed information, please contact the authors directly or via NS (email: metabolonote [at]

Sample Information

Title Blank
Organism - Scientific Name
Organism - ID
Compound - ID
Compound - Source
Sample Preparation Details ID
Comment Methanol [for HPLC Solvent]

Analytical Method Information

Title LC-FTICR-MS, ESI Positive analysis
Method Details ID MS1
Sample Amount
Comment [MassBase ID] MDLC1_05767

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The raw (binary) and near-raw (text) files of this analysis are available at MassBase.

Analytical Method Details Information

Title LC-FTICR-MS, ESI Positive analysis
Instrument LC-FT-ICR-MS; [Agilent 1100 LC (Agilent Technologies); Finnigan LTQ-FT (Thermo Fisher Scientific K.K.)]
Instrument Type LC-FTICR-MS
Ionization ESI
Ion Mode Positive
Description Harvested sample is frozen by liquid N2 and resulting powder (100mg) are solved in 300uL 80% methanol solution. 20uL sample is injected into HPLC after 0.2um membrane filter treatment. HPLC conditions: Agilent 1100 series (Agilent), Solvent: A; 0.1% formic acid aq. B; ACN (addition 0.1% formic acid fc.), Gradient: (B);3 to 40% (0.0 to 23.0 min), 40 to 95% (23.0 to 23.1 min), 95% (23.1 to 25.0 min), 3% (25.1 to 30.0 min), Column temp.: 30 degree C, Flow rate=0.5mL/min, PDA: 200-650 nm (2 nm step). FT-ICR-MS conditions: Filter 1: FTMS + c norm !corona !pi res=50000 o(200.0-1500.0), Rejected mass=266.0000;294.0000;391.0000.
Comment_of_details [MassBase Method ID] MTLC0349
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