SE9:/MS2

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Sample Set Information

ID SE9
Title Marchantia polymorpha metabolite analysis
Description Investigation of Marchantia polymorpha L. Tak-1 (male) metabolites. 2 growth conditions (suc + or -) and 2 replicates data are examined.
Authors Kimitsune Ishizaki 2, Takeshi Ara 1, Daisuke Nakajima 1, Mitsuo Enomoto 1, Nozomu Sakurai 1, Hideyuki Suzuki 1, Koei Okazaki 1, Takayuki Kohchi 2, Daisuke Shibata 1, 1: Kazusa DNA Research Institute, 2: Graduate School of Biosutdies, Kyoto University
Reference Direct Submittion
Comment version 3

Analytical Method Details Information

ID MS2
Title LC-FT-ICR-MS ESI positive method 2
Instrument Agilent1100 HPLC (Agilent), LTQ-FT (Thermo Fisher Scientific)
Instrument Type LC-FTICR-MS
Ionization ESI
Ion Mode Positive
Description Sample is frozen by liquid N2 and resulting powder (250mg) are solved in 750uL 100% methanol solution. Cells are homogenized by TissueLyser (QUIAGEN, 25frequency, 2min) and centrifuged (15000rpm, 10min, 4 degree C). 20uL sample is injected into HPLC after 0.2um membrane filter treatment. HPLC conditions: Agilent 1100 series (Agilent), Column: TSKgel-100V (4.6 x 250 mm, 5 micrometer; TOSOH), Solvent: A; 0.1% formic acid aq. B; ACN (addition 0.1% formic acid fc.), Gradient: (B);3 to 97% (0.0 to 45.0 min), 97% (45.1 to 50.0 min), 3% (50.1 to 57.0 min), Column temp.: 40 degree C, Flow rate=0.5mL/min, PDA: 200-650 nm (2 nm step). FT-ICR-MS conditions: Filter 1: FTMS + c norm !corona !pi res=100000 o(100.0-1500.0); 2: ITMS + c norm !corona !pi Dep MS/MS Most intense ion from (1); 3: ITMS + c norm !corona !pi Dep MS/MS 2nd most intense ion from (1); 4: ITMS + c norm !corona !pi Dep MS/MS 3rd most intense ion from (1); 5: ITMS + c norm !corona !pi Dep MS/MS 4th most intense ion from (1); 6: ITMS + c norm !corona !pi Dep MS/MS 5th most intense ion from (1), Reject Mass List: 235.2000, 376.0400, 609.2800, 810.4100, 1123.6800.
Comment_of_details


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