SE9:/S02/M02

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Sample Set Information

ID SE9
Title Marchantia polymorpha metabolite analysis
Description Investigation of Marchantia polymorpha L. Tak-1 (male) metabolites. 2 growth conditions (suc + or -) and 2 replicates data are examined.
Authors Kimitsune Ishizaki 2, Takeshi Ara 1, Daisuke Nakajima 1, Mitsuo Enomoto 1, Nozomu Sakurai 1, Hideyuki Suzuki 1, Koei Okazaki 1, Takayuki Kohchi 2, Daisuke Shibata 1, 1: Kazusa DNA Research Institute, 2: Graduate School of Biosutdies, Kyoto University
Reference Direct Submittion
Comment version 3


MarpolBase

http://marchantia.info/

Sample Information

ID S02
Title Marchantia polymorpha L. Tak-1 (male)
Organism - Scientific Name Marchantia polymorpha
Organism - ID NCBI taxonomy:3197
Compound - ID
Compound - Source
Preparation Marchantia polymorpha L. Tak-1 (male) are grown on culture plate filled by 1/2Gamborg's B5 culture without sucrose and agar in an incubator. Whole plants are harvested.
Sample Preparation Details ID
Comment [KomicMarket ID] KSBA_31


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The annotation data and quantitative data of the metabolite peaks in this sample are available at KomicMarket.

Analytical Method Information

ID M02
Title LC-FTICR-MS, ESI Positive analysis
Method Details ID MS2
Sample Amount 6.7mg
Comment [MassBase ID] MDLC1_17054


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The raw (binary) and near-raw (text) files of this analysis are available at MassBase.

Analytical Method Details Information

ID MS2
Title LC-FT-ICR-MS ESI positive method 2
Instrument Agilent1100 HPLC (Agilent), LTQ-FT (Thermo Fisher Scientific)
Instrument Type LC-FTICR-MS
Ionization ESI
Ion Mode Positive
Description Sample is frozen by liquid N2 and resulting powder (250mg) are solved in 750uL 100% methanol solution. Cells are homogenized by TissueLyser (QUIAGEN, 25frequency, 2min) and centrifuged (15000rpm, 10min, 4 degree C). 20uL sample is injected into HPLC after 0.2um membrane filter treatment. HPLC conditions: Agilent 1100 series (Agilent), Column: TSKgel-100V (4.6 x 250 mm, 5 micrometer; TOSOH), Solvent: A; 0.1% formic acid aq. B; ACN (addition 0.1% formic acid fc.), Gradient: (B);3 to 97% (0.0 to 45.0 min), 97% (45.1 to 50.0 min), 3% (50.1 to 57.0 min), Column temp.: 40 degree C, Flow rate=0.5mL/min, PDA: 200-650 nm (2 nm step). FT-ICR-MS conditions: Filter 1: FTMS + c norm !corona !pi res=100000 o(100.0-1500.0); 2: ITMS + c norm !corona !pi Dep MS/MS Most intense ion from (1); 3: ITMS + c norm !corona !pi Dep MS/MS 2nd most intense ion from (1); 4: ITMS + c norm !corona !pi Dep MS/MS 3rd most intense ion from (1); 5: ITMS + c norm !corona !pi Dep MS/MS 4th most intense ion from (1); 6: ITMS + c norm !corona !pi Dep MS/MS 5th most intense ion from (1), Reject Mass List: 235.2000, 376.0400, 609.2800, 810.4100, 1123.6800.
Comment_of_details
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