Browse wiki

jump-to-nav Jump to: navigation, search
MS Comment of details Instrument Type: GC-TOF-MS
MS Description Frozen sample was homogenized using mill m Frozen sample was homogenized using mill mixer with liquid nitrogen. Metabolites were extracted by MeOH/H2O/CHCl3. After liquid‐liquid distribution, upper (MeOH/H2O) layer is used as polar fraction and bottom (CHCl3) layer is used as lipid fraction. Ribitol and nonadecanoic acid methyl ester were used for internal standards of polar and lipid fractions. Lipid fraction was methoximated with CHCl3/NaOH/MeOH. After washing by water, CHCl3 layer was dried over by N2 gas. Metabolites were trimethylsilylated with n-Heptane/Pyridine/MSTFA. GC conditions: Agilent 6890N GC (Agilent), Column: DB-17ms (Agilent), Carrier gas: He, MS conditions: Pegasus III TOF-MS system (Leco), mass range: 82 to 500 m/z. system (Leco), mass range: 82 to 500 m/z.
MS ID MS1  +
MS Instrument 6890N GC (Agilent Technologies); Pegasus III TOF-MS system (Leco)  +
MS Ion Mode Positive  +
MS Ionization EI  +
MS Title GC-TOF-MS lipid fraction method  +
Modification dateThis property is a special property in this wiki. 19 May 2020 05:48:43  +
hide properties that link here 
  No properties link to this page.


Enter the name of the page to start browsing from.
Personal tools
View and Edit Metadata