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MS Comment of details [MassBase Method ID] MTLC0316
MS Description Harvested sample is frozen by liquid N2 an Harvested sample is frozen by liquid N2 and resulting powder (100mg) are solved in 300uL 80% methanol solution. 20uL sample is injected into HPLC after 0.2um membrane filter treatment. HPLC conditions: Agilent 1100 series (Agilent), Column: TSKgel-100V (4.6 x 250 mm, 5 micrometer; TOSOH), Solvent: A; 0.1% formic acid aq. B; ACN (addition 0.1% formic acid fc.), Gradient: (B);3 to 30% (0.0 to 25.0 min), 30 to 90% (25.0 to 40.0 min), 90% (40.0 to 45.0 min), 95% (45.1 to 50.0 min), 3% (50.1 to 57.0 min), Column temp.: 30 degree C, Flow rate=0.5mL/min, PDA: 200-650 nm (2 nm step). FT-ICR-MS conditions: Filter 1: FTMS + c norm !corona !pi res=50000 o(200.0-1500.0); 2: ITMS + c norm !corona !pi Dep MS/MS Most intense ion from (1).;3: ITMS + c norm o(200.0-1500.0)., Rejected mass=266.0000;294.0000;391.0000. Rejected mass=266.0000;294.0000;391.0000.
MS ID MS1  +
MS Instrument Agilent1100 HPLC (Agilent), LTQ-FT (Thermo Fisher Scientific)  +
MS Instrument Type LC-FTICR-MS  +
MS Ion Mode Positive  +
MS Ionization ESI  +
MS Title LC-FT-ICR-MS ESI positive method 1  +
Modification dateThis property is a special property in this wiki. 11 September 2021 11:59:33  +
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