SE50:/DS01

Sample Set Information

ID	SE50
Title	Enhancement of oxidative and drought tolerance in arabidopsis by overaccumulation of antioxidant flavonoids
Description	We report that flavonoids with radical scavenging activity mitigate against oxidative and drought stress in Arabidopsis thaliana. Metabolome and transcriptome profiling and experiments with oxidative and drought stress in wild-type, single overexpressors of MYB12/PFG1 (PRODUCTION OF FLAVONOL GLYCOSIDES1) or MYB75/PAP1 (PRODUCTION OF ANTHOCYANIN PIGMENT1), double overexpressors of MYB12 and PAP1, transparent testa4 (tt4) as a flavonoid-deficient mutant, and flavonoid-deficient MYB12 or PAP1 overexpressing lines (obtained by crossing tt4 and the individual MYB overexpressor) demonstrated that flavonoid overaccumulation was key to enhanced tolerance to such stresses. Antioxidative activity assays using 2,2 diphenyl-1-picrylhydrazyl, methyl viologen, and 3,3′-diaminobenzidine clearly showed that anthocyanin overaccumulation with strong in vitro antioxidative activity mitigated the accumulation of reactive oxygen species in vivo under oxidative and drought stress. These data confirm the usefulness of flavonoids for enhancing both biotic and abiotic stress tolerance in crops.
Authors	Ryo Nakabayashi, Keiko Yonekura-Sakakibara, Kaoru Urano, Makoto Suzuki, Yutaka Yamada, Tomoko Nishizawa, Fumio Matsuda, Mikiko Kojima, Hitoshi Sakakibara, Kazuo Shinozaki, Anthony J. Michael, Takayuki Tohge, Mami Yamazak, Kazuki Saito
Reference	Nakabayashi R et al. (2014) The Plant Journal 77: 367-379
Comment

The original paper is available at The Plant Journal.

The raw data files are available at DROP Met web site in PRIMe database of RIKEN.

Data Analysis Details Information

ID	DS01
Title	Untargeted profiling by LC‒QTOF‒MS
Description	The data matrix was aligned using MassLynx ver. 4.1 (Waters). After alignment, de-isotoping, and cutoff of the low-intensity peaks (<500 counts) and the intensity values of the remaining peaks were divided by those of lidocaine ([M + H]+, m/z 235.1804) for normalization. The processed data were used for PCA using SIMCA-P 11.5. The log2-transformed value was used for HCA using MeV 4.8 (http://mev.tm4.org/). All metabolome data acquired by LC‒QTOF‒MS were deposited at DROP Met in PRIMe (http://prime.psc.riken.jp/) and are freely available. These data were also deposited in MetaboLights (accession number MTBLS57) (Steinbeck et al., 2012; Salek et al., 2013) and MetabolomeExpress (Carroll et al., 2010).
Comment_of_details

Related data are deposited in MetaboLights.

The raw data files are available at DROP Met web site in PRIMe database of RIKEN.