SE54:/MS01
From Metabolonote
Sample Set Information
| ID | SE54 |
|---|---|
| Title | Toward genome-wide metabolotyping and elucidation of metabolic system: metabolic profiling of large-scale bioresources |
| Description | We established a novel methodology, termed widely targeted metabolomics, which can generate thousands of metabolome data points in a high-throughput manner. We previously conducted a targeted metabolite analysis of large-scale Arabidopsis bioresources, namely transposon-tagged mutants and accessions, to make a smaller dataset of metabolite accumulation. In this paper, we release approximately 3,000 metabolic profiles obtained by targeted analysis for 36 metabolites and discuss the possible regulation of amino acid accumulation. |
| Authors | Masami Yokota Hirai, Yuji Sawada, Shigehiko Kanaya, Takashi Kuromori, Masatomo Kobayashi, Romy Klausnitzer, Kosuke Hanada, Kenji Akiyama, Tetsuya Sakurai, Kazuki Saito, Kazuo Shinozaki |
| Reference | Hirai YM et al. (2010) Journal of Plant Research 123: 291-298 |
| Comment |
The raw data files are available at DROP Met web site in PRIMe database of RIKEN.
Analytical Method Details Information
| ID | MS01 |
|---|---|
| Title | UPLC-MS |
| Instrument | UPLC (Waters)-quadrupole MS and ZQ mass spectrometers (Waters) |
| Instrument Type | |
| Ionization | ESI |
| Ion Mode | Positive (amino acid analysis) and negative (glucosinolates and flavonoid analysis) |
| Description | A total of 200 seeds of each independent mutant and accession were homogenized using a mixer mill MM 200 (Retsch) in 80 μL of extraction buffer [40% acetonitrile in H2O with 25 μM hydroxyphenyl–glucosinolate (GSL) and 50 μM norleucine as internal standards].The extracts were diluted with 500 lL of LC–MS grade H2O and centrifuged (1,000 g) for 5 min. The supernatants were filtered through a CAPTIVA 0.45μlm filter (Varian). Thirty-six metabolites (17 amino acids, 18 GSLs, and 1 flavonoid; Table S1) were separated on UPLC through a reverse phase column (50 9 2.1 mm,HSS T3 1.8 μm; Waters) (Table S1) and detected using ZQ mass spectrometers (Waters) with an electrospray ionization (ESI) interface (positive mode for amino acid analysis; negative mode for glucosinolates and flavonoid analysis; capillary voltage, +3.0 and -3.0 keV; cone voltage, +25 and -40 V; source temperature, 120℃; desolvation temperature, 350℃; cone gas flow, 50 L/h; desolvation gas flow, 600 L/h). |
| Comment_of_details |
