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The cultivation procedure of Chlamydomonas … The cultivation procedure of Chlamydomonas reinhardtii followed our previous report. The C. reinhardtii CC125 strain was streaked out from cryopreserved stock and cultivated in 75 mL TAP medium in 125 mL shake flasks at 25 °C under constant illumination with cool-white fluorescent bulbs at a fluence rate of 70 µmol m−2 s−1 and with continuous stirring (100 rpm). Four independent cultures were used for this study. The starter culture was harvested at late log-phase and 1 mL cell suspensions were then shifted to 75 mL of fresh TAP medium in 125 mL shake flasks. At 0.2–0.6 OD680 during the late-log phase, 1 mL cell suspensions were injected into 1 mL of –80 °C cold quenching solution composed of 70% methanol in water, centrifuged at 12,000 g for 2 min, and pellets were lyophilized and stored at −80 °C until further analysis. The same quenching procedure was used for all algae strains. procedure was used for all algae strains.
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