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Twenty-eight hybrid aspen trees were grown … Twenty-eight hybrid aspen trees were grown under long photoperiods (long-day conditions, LDs) of 18 h/6 h day/night cycle using photosynthetic active radiation (PAR) light for 12 h at 400 μmol m−2s−1, and extended for another 6 h at 30 μmol m−2s−1. After 3 months, 20 consecutive leaves (the length of the first leaf below the apex was approximately 1 cm) and the apex (defined as apical tissue from which all major leaf primordia had been removed) were sampled from seven plants (LD0-samples). Two days later another seven plants were sampled (LD2-samples), and the daylength was changed to short winter photoperiods (short-day conditions, SDs): 12/12 h day/night). After 2 and 6 days under short photoperiods (SD2 and SD6), seven plants were sampled. <br />
To examine the effect of 35S: : oat PHYA-overexpression, we sampled 10 leaves from 22-PHYAOX and 21-control plants. We used two different photoperiods [LD0 and short photoperiod at day 7 (SD7)] to obtain PHYOX and the control plants (wild-type, WT). After removal from the plant samples were dipped in liquid nitrogen and stored at −80°C until required. trogen and stored at −80°C until required.
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