| MS Description
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Each sample was extracted, derivatized, an … Each sample was extracted, derivatized, and analyzed by using gas chromatography–time-of-flight (GC-TOF/MS) as described in ref. 48. See also SI Methods(http://www.pnas.org/cgi/data/0900952106/DCSupplemental/Supplemental_PDF#nameddest=STXT).<br /><br />
All the derivatization steps were performed in the vacuum glove box VSC-100 (Sanplatec, Japan) filled with 99.9995% (G3 grade) of dry nitrogen. The analysis of metabolites by GC-TOF/MS was performed as described previously. /MS was performed as described previously.
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