| MS Description
|
We used the three different tissues (LB, L … We used the three different tissues (LB, LS and roots) to perform metabolite phenotyping and profiling using GC-TOF-MS (Kusano et al., 2007a). Metabotyping was conducted using six independent seedlings for WT, two independent knockout lines (NC2373 and ND8037) and the corresponding null lines, respectively. Extracts from 5 mg (fresh weight, FW) samples were used for metabotyping. Extracts of 55.6 μg FW samples was injected into the GC-TOF-MS.We performed data pre-treatment and normalisation as described by Kusano et al. (2007a) and Redestig et al. (2009). For metabolite profiling, a total of 16–20 biological replicates were collected,extracted, derivatised and analysed. Extracts from 5 mg FW samples were used. Subsequently, extracts of 83.3 μg FW samples for the ammonium experiment and extracts of 55.6 μg FW samples for the water experiment were injected . s for the water experiment were injected .
|
