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Arabidopsis thaliana ecotype Columbia (Col … Arabidopsis thaliana ecotype Columbia (Col-0) and Nossen were used. Arabidopsis seeds were surface-sterilised and sown on an agar-solidified Murashige and Skoog medium containing 0.5% (w/v) sucrose. Plants were grown at 22 °C under a 16-h-light/8-h-dark cycle. Fourteen-day-old Col-0 and Nossen plants at about 3 h after the onset of the light phase were subjected to heat stress at 22 °C (control), 30 °C, 34 °C or 38 °C for one day under a continuous light condition (biological replicate N = 4) (Supplementary Fig. S1). For recovery experiments, 18-day-old Col-0 and 14-day-old Nossen plants were subjected to heat stress at 38 °C for one day under the 16/8 h light-dark cycle, and then returned to 22 °C and grown for one day and 2 days longer (biological replicate N = 3, 4, 8, or 12).
Aerial parts were harvested at about 3 h after the onset of light phase. about 3 h after the onset of light phase.
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