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Peak areas of individual lipid molecules w … Peak areas of individual lipid molecules were calculated based on the m/z values of their molecular-related ions or fragment ions. The ions used for calculation of peak areas are as follows: [M+HCOO]− for MGDG and DGDG, [M–H]− for SQDG and PI, [M+NH4–phosphoglycerol]+ for PG, [M+NH4–phosphoethanolamine]+ for PE, and [M+H]+ for PC. Because the lipid species with the same polar head were eluted at almost the same retention time (see Supplemental Figure 5 online), corrections for overlap of isotopic variants in higher mass lipids were applied.
Ionization efficiency is largely influenced by the nature of the polar headgroups. Thus, the ionization efficiency is assumed to be almost identical when molecules have the same polar headgroups. Levels of individual lipid molecules in the wild-type plant and mutant lines were expressed as relative values against the sum of the peak areas of lipid molecules with the same polar headgroups in the wild type. he same polar headgroups in the wild type.
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