| MS Description
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Rosette leaves of Arabidopsis (250 mg fres … Rosette leaves of Arabidopsis (250 mg fresh weight) grown in soil were frozen in liquid nitrogen and homogenized to a fine powder using a mortar and pestle. The powder was extracted with 2.5 mL of 50% (v/v) methanol and subjected to UPLC-Q-MS analysis (flow rate, 0.3 mL min−1; column, ACQUITY UPLC HSS T3 1.8 μm [2.1 mm i.d., 50 mm long; Waters]; solvent, 10 mM triethylamine acetate, pH 6.0; column temperature, 30°C; conditions for MS were same as those used for assay of recombinant UGP3) (Ramm et al., 2004). of recombinant UGP3) (Ramm et al., 2004).
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