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Surface-sterilized seeds were sown on agar … Surface-sterilized seeds were sown on agar-solidified Murashige and Skoog medium containing 1% (w/v) sucrose at 22 °C under 16 h light/8 h dark cycles. Photon flux density was 50 μmol m–2 sec–1. The seedlings were kept on agar for 14 days before transfer to plates with controlled phosphate (Pi) concentrations. For P-limitation experiments, sterile Arabidopsis medium was used but at half concentration and containing 0.8% (w/v) agar, 20 mM MES (pH 6.0) and different concentrations of KH2PO4 as indicated. For lipid analysis, leaves were harvested 6 h after the onset of the light phase, frozen in liquid nitrogen and stored at −80 °C until lipid extraction. d stored at −80 °C until lipid extraction.
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