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Tomato seeds (Solanum lycopersicum cv. Mon … Tomato seeds (Solanum lycopersicum cv. Moneymaker) were sterilized by washing with 70% ethanol, followed by gentle agitation in 2% (v/v) plant preserve mixture (PPM; Plant Cell Technology, Jefferson, Washington, D.C., USA) in the dark, overnight, and at room temperature.
Sterilized seeds were incubated for 10 days on wet filter paper at 22°C (18-h light phase/6-h dark phase). Ten-day-old plants were transplanted to rockwool (Grodan) and grown in liquid MGRL medium (Fujiwara et al. 1992). For the P starvation treatment, plants were grown in MGRL medium without potassium phosphate. n MGRL medium without potassium phosphate.
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