| MS Description
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Anthocyanin extraction was carried out in … Anthocyanin extraction was carried out in triplicate as described previously (Tohge et al., 2005). For anthocyanin profiling, Agilent HPLC 1100 series and Agilent single quadrupole LC-MS 6120 series (Agilent Technologies Inc., http://www.home.agilent.com/) were used with an Atlantis® T3 column (Φ4.6 mm × 150 mm, 5 μm, Waters) at a flow rate of 0.5 ml min−1 at 30°C. Anthocyanins were separated with solvent A (10% acetonitrile, 0.1% trifluoroacetic acid in water) and solvent B (90% acetonitrile, 0.1% trifluoroacetic acid in water) using an elution gradient (0 min, 0% B; 40 min, 40% B, 40.1 min, 100% B; 45 min 100% B; 45.1 min, 0% B; 50 min, 0% B). PDA was used for the detection of UV-visible absorption in the range of 200–600 nm. A mass analyzer was used for the detection of anthocyanin glycosides [M]+, and the peak of fragment ions in a positive ion scanning mode with the following setting: drying gas temperature, 350°C with drying gas flow of 12 L/min; capillary voltage, 4.0 kV; nebulizer pressure, 35 psig; fragmentor, 80 V; detection mode, scan (m/z 100–1400). 80 V; detection mode, scan (m/z 100–1400).
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