| MS Description
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Metabolites were extracted by homogenizing … Metabolites were extracted by homogenizing lyophilized callus in 500 uL 80% methanol in 0.1% formic acid per 2 mg dry weight callus with 5 mm zirconia beads (no.5-4060-13, AS ONE Co. Ltd.) in 2.0 mL sampling tubes (no.132-620C, WATSON Co., LTD) for 5 min using shake master NEO (Bio Medical Science, Tokyo, Japan). After centrifugation using a high speed refrigerated micro centrifuge (TOMY MX-300) at 14,000 r.p.m. at 4°C, 250 uL supernatant was dried up in 96 well plate and the residue was dissolved in 120 uL ultrapure water (no. 210-01303, Wako Pure Chemical Industries, Ltd.). One uL of the solution was subjected to widely targeted metabolome analysis by LC-MS using UPLC-TQD system (Waters, Milford, MA, USA). PLC-TQD system (Waters, Milford, MA, USA).
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