| S Preparation
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Seeds of P. hybrida inbred line, V26 (kind … Seeds of P. hybrida inbred line, V26 (kindly provided by Dr. M. Nakayama, NARO Institute of Floricultural Science, Tsukuba, Ibaraki, Japan) were used. Petunia seeds were sown on one-half-strength MS-agar medium containing 2% (w/v) sucrose and placed in a 25 °C growth chamber with a light intensity of 70 µmol of photons m2 s−1 and a 16 h light/8 h dark photoperiod. After 8 weeks, the seedlings were transferred to sterile vermiculite and acclimated. After acclimation, plants were transferred to soil and grown for 3 months in a greenhouse. il and grown for 3 months in a greenhouse.
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