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The MS spectra were recorded using Hystar … The MS spectra were recorded using Hystar 4.0 (Bruker Daltonik GmbH, Bremen, Germany) and the data were processed using DataAnalysis 4.2 (Bruker Daltonik GmbH). Internal calibration was performed using the exact mass of the internal standards. Molecular formulae were determined from an in-house database storing metabolite information downloaded on April 1, 2015, from metabolome databases: [(BMDB (http://www.cowmetdb.ca/cgi-bin/browse.cgi) (2697), ChEBI (Hastings et al. 2013) (14,546), DrugBank (Wishart et al. 2006) (5355), ECMDB (Guo et al. 2013) (987), FooDB (http://foodb.ca/) (6441), HMDB (Wishart et al. 2007) (9652), KNApSAcK (Afendi et al. 2012) (13,825), PlantCyc (http://www.plantcyc.org/) (2421), PubChem Classification Browser (Biosystems and Pathways, https://pubchem.ncbi.nlm.nih.gov/classification/#hid=72) (8242), SMPDB (Frolkis et al. 2010) (1187), T3DB (Lim et al. 2010) (1726), UNPD (http://pkuxxj.pku.edu.cn/UNPD/) (32,952), and YMDB (Jewison et al. 2012) (1047)]. The number of molecular formulae downloaded from each database is shown in the parentheses. A personal computer (Windows 10 Intel Xeon (R) CPU E5-2650 v3 (2.3 GHz) with 128 GB RAM) required around 1.5 min to analyze a pair of the non-labeled and 34S-labeled data of a certain organ. The program named SMetSearch can be freely downloaded from the standalone software section of the RIKEN PRIMe database (http://prime.psc.riken.jp/Metabolomics_Software/SmetSearch/index.html). abolomics_Software/SmetSearch/index.html).
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