| MS Description
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Equal amounts of cells (50 ml of cell cult … Equal amounts of cells (50 ml of cell culture with A730 = 1.0) were harvested by rapid filtration using a previously described method (Osanai et al., 2014b). In total, 300 μl of the upper phase was transferred to a new tube and vacuum dried. Samples were suspended in 500 μl methanol with 1 μM norvaline as an internal standard and derivatized using the EZ:faast kit (Phenomenex, CA, USA) (Badawy et al., 2008). GC‐MS was performed using GCMS‐QP2010Plus (Shimadzu, Kyoto, Japan) equipped with a 10 m × 0.25 mm ZB‐AAA capillary GC column. The injection volume was 1 μl at a carrier gas flow of 1.17 ml min−1 helium with a split ratio of 1:15. The initial column oven temperature of 110°C was maintained for 1 min and raised to 300°C at 20°C min−1. The interface and ion source temperatures were 280°C and 240°C respectively. ratures were 280°C and 240°C respectively.
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