| S Preparation
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Cultivation of cyanobacterial strains was … Cultivation of cyanobacterial strains was performed as previously described (Osanai et al., 2013a). The GT strain of Synechocystis sp. PCC 6803, isolated by Williams (1988), was grown in modified BG-11 medium (Rippka, 1988), containing 5 mM NH4Cl, instead of 17.5 mM NaNO3 (buffered with 20 mM Hepes-KOH, pH 7.8). Of the GT substrains, the GT-I strain was used in this study (Kanesaki et al., 2012). Liquid cultures were bubbled with 1% (v/v) CO2 in air at 30°C under continuous white light (approximately 50–70 μmol photons m−2 s−1). Growth and cell densities were measured at A730 with a Hitachi U-3310 spectrophotometer (Hitachi, Tokyo, Japan). To create nitrogen-starved conditions, cells were collected by filtration with mixed-cellulose ester (Advantec, Tokyo, Japan) and resuspended in BG-110 liquid medium. ) and resuspended in BG-110 liquid medium.
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