| MS Description
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Equal amounts of cells (10 mL cell culture … Equal amounts of cells (10 mL cell culture with A730 = 1.0) were harvested by rapid filtration, and metabolites were extracted using a previously described method (Osanai et al., 2014b). Briefly, the cells were filtrated, and then the intermediate metabolites were quenched and extracted in 1.2 mL of solvent mixture (CHCl3:CH3OH:H2O, 2.5:2.5:1, v/v/v) containing 10 μg/L D-(+)-camphor-10-sulfonic acid as an internal standard. After centrifugation at 15,000 × g at 4°C for 5 min, 400 μL of the upper phase was transferred to a new tube and vacuum-dried. ransferred to a new tube and vacuum-dried.
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