| MS Description
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Freeze-dried supernatants were resolved in … Freeze-dried supernatants were resolved in 100 μL of filtered 3 mM perchloric acid. The resolved samples were analyzed by HPLC using a LC-2000Plus Systems (JASCO, Tokyo, Japan) with a photodiode array detector and two RSpak KC-811 columns (Showa Denko, Tokyo, Japan). Organic acids were quantified with 0.2 mM bromothymol blue in 15 mM sodium phosphate buffer; peaks were detected at 445 nm. The column temperature was 60°C, and the flow rates of 3 mM perchloric acid and 0.2 mM bromothymol blue solutions were 1.0 and 1.5 mL/min, respectively. ons were 1.0 and 1.5 mL/min, respectively.
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